How to Collect Water Samples - Pool and Spa

Follow these steps to collect and send pool or spa water samples for bacteria testing.

Collecting and handling your samples the right way helps the lab give you accurate results.

How to Collect a Pool Sample


Water Collection Steps

Step 1.Wash your hands

Wash your hands well before you collect samples.

Step 2.Choose when and where to collect

Collect samples where the most people swim, or at the time of day when the most people are in the water.

Step 3.Get your bottles ready

Each pool or spa needs 2 bottles:

  • 1 bottle to test for total coliform bacteria
  • 1 bottle to test for Pseudomonas bacteria

Step 4.Remove the cap carefully

Hold the bottle by its base, tilted at a 45-degree angle. Remove the cap.

  • Do not touch the inside of the cap with your fingers.
  • Do not set the cap down on any surface.

Step 5.Fill the bottle

Fill the bottle to the fill line or just above it. Do this with one slow sweep under the surface of the water.

Push the bottle forward through the water so the opening always moves ahead of your hand.

Caution: The inside of the bottle is sterile (germ-free). It contains sodium thiosulfate, a chemical that neutralizes chlorine and bromine. Do not put your fingers inside the bottle, and do not rinse it out.

Step 6.Fill directly from the pool or spa

Do not use a cup, container, or hose to fill the bottle.

Step 7.Put the cap back on

Tighten the cap so the bottle does not leak. Do not overtighten, because that can crack the cap or bottle.

Step 8.Label the bottle

Write on the bottle, not the cap. Label it with the type of water you collected from, such as pool or spa.

Step 9.Fill out the requisition form

Complete the water testing data form with as much information as possible.

Step 10.Send your samples to the lab

Mail or deliver your samples as soon as possible. The lab must receive them within 24 hours of collection.
*We do not collect pool samples on Fridays